ANKRD50 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50032
![Western Blot: ANKRD50 Antibody [NBP1-50032]](https://resources.bio-techne.com/images/products/ANKRD50-Antibody-Western-Blot-NBP1-50032-img0002.jpg "Western Blot: ANKRD50 Antibody [NBP1-50032]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Canine (100%), Equine (100%), Guinea Pig (100%), Monkey (100%), Porcine (100%), Primate (100%), Rabbit (100%), Xenopus (100%). Backed by our 100% Guarantee.
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Summary for ANKRD50 Antibody
Immunogen
The immunogen for this product maps to a region between residue 1379 and 1429 of human Ankyrin Repeat Domain 50 using the numbering given in entry NP_065070.1 (GeneID 57182).
Reactivity Notes
Panda, Orangutan, Gorilla and Chimpanzee (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ANKRD50 Antibody
Western Blot: ANKRD50 Antibody [NBP1-50032]
Western Blot: ANKRD50 Antibody [NBP1-50032] - Detection of Human and Mouse ANKRD50 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-ANKRD50 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ANKRD50 was also immunoprecipitated by rabbit anti-ANKRD50 antibody which recognizes an upstream epitope. For blotting immunoprecipitated ANKRD50, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).Applications for ANKRD50 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ANKRD50
Alternate Names
ankyrin repeat domain 50
Gene Symbol
ANKRD50
Additional ANKRD50 Products
Product Documents for ANKRD50 Antibody
Product Specific Notices for ANKRD50 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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