ANKRD50 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-93594

Novus Biologicals, part of Bio-Techne
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NBP1-93594
NBP1-93594-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ANKRD50 Antibodies »

Product Summary for ANKRD50 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CKIMIPSAQQEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTMQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFN

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

156 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ANKRD50 Antibody

Western Blot: ANKRD50 Antibody [NBP1-93594]

Western Blot: ANKRD50 Antibody [NBP1-93594]

Western Blot: ANKRD50 Antibody [NBP1-93594] - Analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: ANKRD50 Antibody [NBP1-93594]

Immunocytochemistry/ Immunofluorescence: ANKRD50 Antibody [NBP1-93594]

Immunocytochemistry/Immunofluorescence: ANKRD50 Antibody [NBP1-93594] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: ANKRD50 Antibody [NBP1-93594]

Immunohistochemistry-Paraffin: ANKRD50 Antibody [NBP1-93594]

Immunohistochemistry-Paraffin: ANKRD50 Antibody [NBP1-93594] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
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Applications for ANKRD50 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200-1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ANKRD50

Alternate Names

ankyrin repeat domain 50

Entrez Gene IDs

57182 (Human)

Gene Symbol

ANKRD50

UniProt

Q9ULJ7 (Human)

Additional ANKRD50 Products

Product Documents for ANKRD50 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ANKRD50 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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