ANKRD50 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-93594
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CKIMIPSAQQEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTMQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFN
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
156 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ANKRD50 Antibody
Western Blot: ANKRD50 Antibody [NBP1-93594]
Western Blot: ANKRD50 Antibody [NBP1-93594] - Analysis in human cell line RT-4.Immunocytochemistry/ Immunofluorescence: ANKRD50 Antibody [NBP1-93594]
Immunocytochemistry/Immunofluorescence: ANKRD50 Antibody [NBP1-93594] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.Immunohistochemistry-Paraffin: ANKRD50 Antibody [NBP1-93594]
Immunohistochemistry-Paraffin: ANKRD50 Antibody [NBP1-93594] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.Applications for ANKRD50 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200-1:500
Immunohistochemistry-Paraffin
1:200-1:500
Western Blot
1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ANKRD50
Additional ANKRD50 Products
Product Documents for ANKRD50 Antibody
Product Specific Notices for ANKRD50 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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