ANKRD53 Antibody (OTI1E1) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72217
![Flow Cytometry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217]](https://resources.bio-techne.com/images/products/ANKRD53-Antibody-OTI1E1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72217-img0005.jpg "Flow Cytometry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217]")
![Western Blot: ANKRD53 Antibody (OTI1E1)Azide and BSA Free [NBP2-72217]](https://resources.bio-techne.com/images/products/ANKRD53-Antibody-OTI1E1-Azide-and-BSA-Free-Western-Blot-NBP2-72217-img0002.jpg "Western Blot: ANKRD53 Antibody (OTI1E1)Azide and BSA Free [NBP2-72217]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI1E1
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-300 of human ANKRD53 (NP_079209) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ANKRD53 Antibody (OTI1E1) - Azide and BSA Free
Western Blot: ANKRD53 Antibody (OTI1E1)Azide and BSA Free [NBP2-72217]
Western Blot: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANKRD53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANKRD53.![Immunocytochemistry/ Immunofluorescence: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217]](https://resources.bio-techne.com/images/products/ANKRD53-Antibody-OTI1E1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-72217-img0006.jpg "Immunocytochemistry/ Immunofluorescence: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217]")
Immunocytochemistry/ Immunofluorescence: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217]
Immunocytochemistry/Immunofluorescence: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ANKRD53.![Immunohistochemistry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217]](https://resources.bio-techne.com/images/products/ANKRD53-Antibody-OTI1E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72217-img0007.jpg "Immunohistochemistry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217]")
Immunohistochemistry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217]
Immunohistochemistry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217] - Staining of paraffin-embedded Human lymph node tissue using anti-ANKRD53 mouse monoclonal antibody.![Western Blot: ANKRD53 Antibody (OTI1E1)Azide and BSA Free [NBP2-72217]](https://resources.bio-techne.com/images/products/ANKRD53-Antibody-OTI1E1-Azide-and-BSA-Free-Western-Blot-NBP2-72217-img0001.jpg "Western Blot: ANKRD53 Antibody (OTI1E1)Azide and BSA Free [NBP2-72217]")
![Immunohistochemistry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217]](https://resources.bio-techne.com/images/products/ANKRD53-Antibody-OTI1E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72217-img0008.jpg "Immunohistochemistry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217]")
![Flow Cytometry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217]](https://resources.bio-techne.com/images/products/ANKRD53-Antibody-OTI1E1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72217-img0003.jpg "Flow Cytometry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217]")
![Flow Cytometry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217]](https://resources.bio-techne.com/images/products/ANKRD53-Antibody-OTI1E1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72217-img0004.jpg "Flow Cytometry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217]")
Applications for ANKRD53 Antibody (OTI1E1) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:500-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ANKRD53
Alternate Names
ankyrin repeat domain 53, ankyrin repeat domain-containing protein 53, FLJ12056, FLJ36160
Gene Symbol
ANKRD53
Additional ANKRD53 Products
Product Documents for ANKRD53 Antibody (OTI1E1) - Azide and BSA Free
Product Specific Notices for ANKRD53 Antibody (OTI1E1) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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