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ANKS3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83531

Novus Biologicals, part of Bio-Techne
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NBP1-83531
NBP1-83531-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: REEHAFCANLGPVQSSSSSEGLARAQGLSSEASVESNEDSDHACKSSARKQAKSYMKTKNPDSQWPPRTATDREGFLAESSPQTQRAPYSGPQD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ANKS3 Antibody

Western Blot: ANKS3 Antibody [NBP1-83531]

Western Blot: ANKS3 Antibody [NBP1-83531]

Western Blot: ANKS3 Antibody [NBP1-83531] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: ANKS3 Antibody [NBP1-83531]

Immunocytochemistry/ Immunofluorescence: ANKS3 Antibody [NBP1-83531]

Immunocytochemistry/Immunofluorescence: ANKS3 Antibody [NBP1-83531] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: ANKS3 Antibody [NBP1-83531]

Immunohistochemistry-Paraffin: ANKS3 Antibody [NBP1-83531]

Immunohistochemistry-Paraffin: ANKS3 Antibody [NBP1-83531] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.

Applications for ANKS3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ANKS3

ANKS3 (ankyrin repeat and SAM domain-containing protein 3) contains 6 ANK (ankyrin) repeats and a SAM (sterile alpha motif) domain. Each ANK repeat consists of a 30-34 amino acid sequence that exhibits a helix-turn-helix conformation and functions to mediate protein-protein interactions. The SAM domain is a 70 amino acid domain that also functions to mediate protein-protein interactions for homo- and hetero- oligomerization. The function of ANKS3 has not been characterized.

Alternate Names

ankyrin repeat and SAM domain-containing protein 3, ankyrin repeat and sterile alpha motif domain containing 3, FLJ32767, KIAA1977FLJ32345

Gene Symbol

ANKS3

Additional ANKS3 Products

Product Documents for ANKS3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ANKS3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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