Ankyrin 1 Antibody (3C3)

Novus Biologicals, part of Bio-Techne | Catalog # H00000286-M01

Novus Biologicals, part of Bio-Techne
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H00000286-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3C3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ankyrin 1 Antibodies »

Product Specifications

Immunogen

ANK1 (AAH30957, 1 a.a. ~ 155 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MWTFVTQLLVTLVLLSFFLVSCQNVMHIVRGSLCFVLKHIHQELDKELGESEDLSDDEETISTRVVRRRVFLKGNEFQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQIDLSSADAAQEHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

Specificity

ANK1 - ankyrin 1, erythrocytic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Ankyrin 1 Antibody (3C3)

Immunoprecipitation: Ankyrin 1 Antibody (3C3) [H00000286-M01]

Immunoprecipitation: Ankyrin 1 Antibody (3C3) [H00000286-M01]

Immunoprecipitation: Ankyrin 1 Antibody (3C3) [H00000286-M01] - Analysis of ANK1 transfected lysate using anti-ANK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ANK1 MaxPab rabbit polyclonal antibody.
ELISA: Ankyrin 1 Antibody (3C3) [H00000286-M01]

ELISA: Ankyrin 1 Antibody (3C3) [H00000286-M01]

ELISA: Ankyrin 1 Antibody (3C3) [H00000286-M01] - Detection limit for recombinant GST tagged ANK1 is 0.3 ng/ml as a capture antibody.

Applications for Ankyrin 1 Antibody (3C3)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ankyrin 1

Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known. Alternative polyadenylation accounting for the different sized erythrocytic ankyrin 1 mRNAs, has also been reported. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.

Alternate Names

ANK, ANK-1, ankyrin 1, erythrocytic, ankyrin-1, ankyrin-R, Erythrocyte ankyrin, SPH1, SPH2

Entrez Gene IDs

286 (Human)

Gene Symbol

ANK1

OMIM

182900 (Human)

UniProt

AAH30957 (Human)

Additional Ankyrin 1 Products

Product Documents for Ankyrin 1 Antibody (3C3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ankyrin 1 Antibody (3C3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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