Ankyrin 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33806

Novus Biologicals, part of Bio-Techne
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NBP2-33806
NBP2-33806-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ankyrin 1 Antibodies »

Product Summary for Ankyrin 1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: FVLVSDKHRMSFPETVDEILDVSEDEGEELISFKAERRDSRDVDEEKELLDFVPKLDQVVESPAIPRIPCAMPETVVIRSEEQEQASKEYDEDSLIPSSP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ankyrin 1 Antibody

Western Blot: Ankyrin 1 Antibody [NBP2-33806]

Western Blot: Ankyrin 1 Antibody [NBP2-33806]

Western Blot: Ankyrin 1 Antibody [NBP2-33806] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: Ankyrin 1 Antibody [NBP2-33806]

Immunohistochemistry-Paraffin: Ankyrin 1 Antibody [NBP2-33806]

Immunohistochemistry-Paraffin: Ankyrin 1 Antibody [NBP2-33806] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Ankyrin 1 Antibody

Immunohistochemistry-Paraffin: Ankyrin 1 Antibody [NBP2-33806] -

Immunohistochemistry-Paraffin: Ankyrin 1 Antibody [NBP2-33806] - Staining of human cerebellum shows weak membranous and cytoplasmic positivity in Purkinje cells.
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Applications for Ankyrin 1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ankyrin 1

Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actincytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and themaintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various targetproteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed ofthree structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highlyconserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subjectto variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has alsobeen found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of allpatients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving riseto different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting fromusage of an alternate promoter have also been identified. (provided by RefSeq)

Alternate Names

ANK, ANK-1, ankyrin 1, erythrocytic, ankyrin-1, ankyrin-R, Erythrocyte ankyrin, SPH1, SPH2

Gene Symbol

ANK1

UniProt

P16157 (Human)

Additional Ankyrin 1 Products

Product Documents for Ankyrin 1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ankyrin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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