Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S106-20

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ankyrin 3 Antibodies »

Product Specifications

Immunogen

Fusion protein 1000 C-terminal amino acids of human Ankyrin 3 (also known as ANK-3 or Ankyrin G) encompassing all of Ankyrin 3 with the exception of Ankyrin repeats.

Localization

Cytoplasm , Cytoskeleton , Cell Projection , Axon , Cell Membrane , Sarcolemma , Cell Junction , Synapse , Postsynaptic Cell Membrane , Lysosome

Specificity

Detects >200kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Ankyrin 3 Antibody (S106-20)

Western Blot: Ankyrin 3 Antibody (S106-20) [NBP2-59310]

Western Blot: Ankyrin 3 Antibody (S106-20) [NBP2-59310]

Western Blot: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Western Blot analysis of Rat brain membrane lysate showing detection of Ankyrin 3 protein using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:1000.
Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310]

Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310]

Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin 3 Monoclonal Antibody, Clone S106-20 (NBP2-59310). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin 3 Monoclonal Antibody (NBP2-59310) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin 3 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310]

Immunocytochemistry/ Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310]

Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody (S106-20) [NBP2-59310] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin G Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X.
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Applications for Ankyrin 3 Antibody (S106-20)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Ankyrin 3

Alternate Names

ankyrin G, Ankyrin G119, ankyrin-3, ankyrin-G, Node Of Ranvier

Gene Symbol

ANK3

Additional Ankyrin 3 Products

Product Documents for Ankyrin 3 Antibody (S106-20)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ankyrin 3 Antibody (S106-20)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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