Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S105-13

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ankyrin Brain Antibodies »

Product Summary for Ankyrin Brain Antibody (S105-13)

Immunogen

Synthetic peptide amino acids 203-496 of human Ankyrin-B

Localization

Cytoplasm , Cytoskeleton , Myofibril , Sarcomere , M line , Apical Cell Membrane , Cell Membrane

Specificity

Detects 200kDa. No cross reactivity against Ankyrin-G.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Ankyrin Brain Antibody (S105-13)

Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374]

Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374]

Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ankyrin Brain Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374]

Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374]

Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm
Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374]

Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374]

Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody (S105-13) [NBP2-59374] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin Brain Monoclonal Antibody, Clone S105-13 (NBP2-59374). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin Brain Monoclonal Antibody (NBP2-59374) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm

Applications for Ankyrin Brain Antibody (S105-13)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:200

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Ankyrin Brain

Alternate Names

Ank2, Ankyrin 2, Ankyrin 2 brain, Ankyrin B, Ankyrin brain, Ankyrin nonerythroid, Brain Ankyrin

Gene Symbol

ANK2

Additional Ankyrin Brain Products

Product Documents for Ankyrin Brain Antibody (S105-13)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Ankyrin Brain Antibody (S105-13)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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