Ankyrin Brain Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33863

Novus Biologicals, part of Bio-Techne
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NBP2-33863
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ankyrin Brain Antibodies »

Product Summary for Ankyrin Brain Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGKHATDTNLVECLTKINRMDIVHLMETNTEPLQERISHSYAEIEQTITLDHSEGFSVLQEELCTAQHKQKEEQAVSKESETC

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ankyrin Brain Antibody

Western Blot: Ankyrin Brain Antibody [NBP2-33863]

Western Blot: Ankyrin Brain Antibody [NBP2-33863]

Western Blot: Ankyrin Brain Antibody [NBP2-33863] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody [NBP2-33863]

Immunocytochemistry/ Immunofluorescence: Ankyrin Brain Antibody [NBP2-33863]

Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cell line RH-30 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863]

Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863]

Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human placenta shows no positivity in trophoblastic cells as expected.
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Applications for Ankyrin Brain Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-P Retrieval method: HIER pH6. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ankyrin Brain

Alternate Names

Ank2, Ankyrin 2, Ankyrin 2 brain, Ankyrin B, Ankyrin brain, Ankyrin nonerythroid, Brain Ankyrin

Gene Symbol

ANK2

UniProt

Q01484 (Human)

Additional Ankyrin Brain Products

Product Documents for Ankyrin Brain Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ankyrin Brain Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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