Annexin A1 Antibody (2F1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23485

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F1

Concentration

1.0 mg/ml

View all Annexin A1 Antibodies »

Product Specifications

Immunogen

Partial recombinant human Annexin A1 (between residues 100-300) expressed in E. coli [UniProt P04083]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Annexin A1 Antibody (2F1)

Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485]

Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485]

Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485] - WB analysis of ANXA1 in (1) Hela, (2) A549, (3) K562, (4) NIH3T3, (5) C6, and (6) COS7 cell lysate.
Immunohistochemistry: Annexin A1 Antibody (2F1) [NBP2-23485]

Immunohistochemistry: Annexin A1 Antibody (2F1) [NBP2-23485]

Immunohistochemistry: Annexin A1 Antibody (2F1) [NBP2-23485] - IHC analysis of paraffin embedded human cervical cancer tissues with DAB staining.
Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485]

Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485]

Western Blot: Annexin A1 Antibody (2F1) [NBP2-23485] - WB detection of ANXA1 in human ANXA1 recombinant protein.

Applications for Annexin A1 Antibody (2F1)

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 30045979)

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-23485 in the following applications:

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS, 0.5% protein stabilizer

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Annexin A1

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq]

Alternate Names

ANX1, ANXA1, LPC1

Entrez Gene IDs

301 (Human); 16952 (Mouse)

Gene Symbol

ANXA1

UniProt

P04083, P10107 (Mouse)

Additional Annexin A1 Products

Product Documents for Annexin A1 Antibody (2F1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Annexin A1 Antibody (2F1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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