Annexin A1 Antibody (CL0199)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52875

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-52875
NBP2-52875-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0199

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Annexin A1 Antibodies »

Product Summary for Annexin A1 Antibody (CL0199)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: FRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMV

Epitope

PQLRRVFQKY

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (86%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Annexin A1 Antibody (CL0199)

Western Blot: Annexin A1 Antibody (CL0199) [NBP2-52875]

Western Blot: Annexin A1 Antibody (CL0199) [NBP2-52875]

Western Blot: Annexin A1 Antibody (CL0199) [NBP2-52875] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-ANXA1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Annexin A1 Antibody (CL0199) [NBP2-52875]

Immunocytochemistry/ Immunofluorescence: Annexin A1 Antibody (CL0199) [NBP2-52875]

Immunocytochemistry/Immunofluorescence: Annexin A1 Antibody (CL0199) [NBP2-52875] - Staining of A549 cells using the Anti-ANXA1 monoclonal antibody, showing specific staining in the nucleus, cytosol and the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]

Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]

Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875] - Staining of human placenta shows strong immunoreactivity in the trophoblast cells.
View 5 more images

Applications for Annexin A1 Antibody (CL0199)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Annexin A1

Annexin I, also known as Annexin A1, belongs to the annexin family of calcium dependent phospholipid binding proteins.

Alternate Names

ANX1, ANXA1, LPC1

Gene Symbol

ANXA1

Additional Annexin A1 Products

Product Documents for Annexin A1 Antibody (CL0199)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Annexin A1 Antibody (CL0199)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.