Annexin A1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33684
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (90%), Canine (91%), Primate (100%), Rabbit (91%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary.
Reactivity Notes
Chimpanzee (100%).
Localization
Nucleus, Cytoplasm, Cell projection, cilium, Basolateral cell membrane
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Annexin A1 Antibody
Knockout Validated: Annexin A1 Antibody [NBP1-33684]
Knockout Validated: Annexin A1 Antibody [NBP1-33684] - Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Western Blot: Annexin A1 Antibody [NBP1-33684]
Western Blot: Annexin A1 Antibody [NBP1-33684] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: Annexin A1 Antibody [NBP1-33684]
Immunocytochemistry/Immunofluorescence: Annexin A1 Antibody [NBP1-33684] - Annexin A1 antibody detects Annexin A1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Annexin A1 stained by Annexin A1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.Applications for Annexin A1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Annexin A1
Alternate Names
ANX1, ANXA1, LPC1
Gene Symbol
ANXA1
Additional Annexin A1 Products
Product Documents for Annexin A1 Antibody
Product Specific Notices for Annexin A1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.