Annexin A2 Antibody (M009)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23188
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M009
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone M009 was generated from a proprietary antigen related to human Annexin A2 in MDA-MB-231 breast cancer cell line. [UniProt# P07355]
Specificity
Clone M009 detects a 36 kDa protein corresponding to the molecular mass of Annexin A2 on SDS-PAGE immunoblots of human A431, MDA-MB-231, MeWo, HUVEC, and PC3 cells. Mass spectrometry analysis of immunoprecipitates using AM0091 in human A431 cell lysates confirms that this antibody only detects Annexin A2 protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Annexin A2 Antibody (M009)
Western Blot: Mouse Annexin A2 mAb (M009) [NBP3-23188]
Western Blot: Mouse Annexin A2 mAb (M009) [NBP3-23188] - Analysis of human A431 (lane 1), MDA-MB-231 (lane 2), LNCaP (lane 3), MeWo (lane 4), HUVEC (lane 5), Jurkat (lane 6), K562 (lane 7), and PC3 (lane 8) cell lysates. The blot was probed with Mouse Annexin A2 mAb (M009) at 1:500 (lanes 1-8).
Immunocytochemistry/Immunofluorescence: Mouse Annexin A2 mAb (M009) [NBP3-23188]
Immunocytochemistry/Immunofluorescence: Mouse Annexin A2 mAb (M009) [NBP3-23188] - Labeling of Annexin A2 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Annexin A2 (clone M009). The antibody was detected using goat anti-mouse DyLight® 594.Applications for Annexin A2 Antibody (M009)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunoprecipitation
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Annexin A2
Alternate Names
ANX2L4, ANXA2, CAL1H, LIP2, Lipocortin-2, LPC2D, PAP-IV
Gene Symbol
ANXA2
Additional Annexin A2 Products
Product Documents for Annexin A2 Antibody (M009)
Product Specific Notices for Annexin A2 Antibody (M009)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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