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Annexin A2 Antibody (M298)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23206

Novus Biologicals, part of Bio-Techne
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NBP3-23206

Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M298

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Clone M009 was generated from a proprietary antigen related to human Annexin A2 in MDA-MB-231 breast cancer cell line. [UniProt# P07355]

Specificity

This antibody detects a 36 kDa protein corresponding to the molecular mass of Annexin A2 on SDS-PAGE immunoblots of rat PC12 cells. The antibody has been shown to label Annexin A2 in immunocytochemical analysis of PC12 cells and immunohistochemical analysis of mouse diaphragm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Annexin A2 Antibody (M298)

Annexin A2 Antibody (M298)

Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (M298) [NBP3-23206] -

Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (M298) [NBP3-23206] - Immunocytochemical labeling of Annexin A2 in NGF-differentiated rat PC12 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40, then labeled with mouse monoclonal Annexin A2 (C-terminal region). The antibody was detected using goat anti-mouse DyLight(R) 594.
Annexin A2 Antibody (M298)

Western Blot: Annexin A2 Antibody (M298) [NBP3-23206] -

Western Blot: Annexin A2 Antibody (M298) [NBP3-23206] - Western blot of rat PC12 cell lysate. The blot was probed with mouse monoclonal anti-Annexin A2 antibody at 1:250 (lane 1) or 1:1000 (lane 2).

Applications for Annexin A2 Antibody (M298)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:50

Immunoprecipitation

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Annexin A2

The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin II is a calcium regulated, membrane binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ANX2L4, ANXA2, CAL1H, LIP2, Lipocortin-2, LPC2D, PAP-IV

Gene Symbol

ANXA2

Additional Annexin A2 Products

Product Documents for Annexin A2 Antibody (M298)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Annexin A2 Antibody (M298)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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