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Annexin A7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31442

Novus Biologicals, part of Bio-Techne
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NBP1-31442

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (93%), Chimpanzee (99%), Mouse (94%), Rat (94%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Annexin A7. The exact sequence is proprietary.

Reactivity Notes

Chicken (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Annexin A7 Antibody

Western Blot: Annexin A7 Antibody [NBP1-31442]

Western Blot: Annexin A7 Antibody [NBP1-31442]

Western Blot: Annexin A7 Antibody [NBP1-31442] - A. 30 ug 293T whole cell extractB. 30 ug A431 whole cell extractC. 30 ug HeLa whole cell extractD. 30 ug HepG2 whole cell extract10 % SDS-PAGE Annexin VII antibody dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: Annexin A7 Antibody [NBP1-31442]

Immunocytochemistry/ Immunofluorescence: Annexin A7 Antibody [NBP1-31442]

Immunocytochemistry/Immunofluorescence: Annexin A7 Antibody [NBP1-31442] - Sample: HeLa cells were fixed in iced-cold MeOH for 5 min. Green: ANXA7 protein stained by Annexin VII antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: Annexin A7 Antibody [NBP1-31442]

Immunohistochemistry-Paraffin: Annexin A7 Antibody [NBP1-31442]

Immunohistochemistry-Paraffin: Annexin A7 Antibody [NBP1-31442] - Paraffin-embedded human gastric carcinoma dilution: 1:500.

Applications for Annexin A7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Annexin A7

Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion.

Alternate Names

ANX7, ANXA7, Synexin

Gene Symbol

ANXA7

Additional Annexin A7 Products

Product Documents for Annexin A7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Annexin A7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.