Anthrax PA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56670

Novus Biologicals, part of Bio-Techne
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NB100-56670
NB100-56670SS
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Azide Free
NB100-55743
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Key Product Details

Species Reactivity

Bacteria

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Anthrax PA Antibodies »

Product Specifications

Immunogen

The antibody was developed by immunizing rabbits with synthetic peptides corresponding to amino acids 186-192 of the Anthrax PA protein (approx. 83 kDa).

Reactivity Notes

Anthrax (Bacillus anthracis) Protective Antigen (PA)

Specificity

PA is cleaved from the 83 kDa form to the 63 kDa form at amino acids 164-167. Thus the antibody (made against amino acids 186-192) should recognize both the 83 kDa and 63 kDa PA forms, but not the 20 kDa cleavage fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Anthrax PA Antibody

Western Blot: Anthrax PA Antibody [NB100-56670]

Western Blot: Anthrax PA Antibody [NB100-56670]

Western Blot: Anthrax PA Antibody [NB100-56670] - Analysis using the Azide Free version of NB100-56670. Detection of Anthrax PA in recombinant protein using this antibody. Lane A shows the pre-bleed.
Western Blot: Anthrax PA Antibody [NB100-56670]

Western Blot: Anthrax PA Antibody [NB100-56670]

Western Blot: Anthrax PA Antibody [NB100-56670] - Analysis using the Azide Free version of NB100-56670. Detection of Anthrax PA in recombinant protein using this antibody (Lane B) at 1:1000 dilution. Lane A shows the pre-bleed.

Applications for Anthrax PA Antibody

Application
Recommended Usage

Western Blot

2 ug/ml to 10 ug/ml

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole Rabbit antisera diluted with PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Anthrax PA

Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxic entry into cells. PA is secreted from Bacillus anthracis as an 83 kDa (PA83) protein which is cleaved by furin protease, releasing a 20 kDa fragment. The 63 kDa portion (PA63) of the cleavage product forms a heptamer, which binds the toxins LF and EF. This complex enters via receptor-mediated endocytosis where LF and EF exert their toxic effects.

Alternate Names

anthrax PA, Anthrax toxins translocating protein, Bacillus anthracis protective antigen, PA, PA 83, PA83, PAG, Paga, Protective antigen

Additional Anthrax PA Products

Product Documents for Anthrax PA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Anthrax PA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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