Anti- Adalimumab (Anti-Idiotype) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9546
Key Product Details
Species Reactivity
Multi-Species
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 972557
Product Specifications
Immunogen
Full length Adalimumab
Specificity
Detects Adalimumab in Western blots and direct ELISAs. In direct ELISAs, no cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Trastuzumab, Tositumomab, Brentuximab, Gemtuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Anti- Adalimumab (Anti-Idiotype) Antibody
Detection of Adalimumab by Western Blot.
Western blot shows Trastuzumab (negative control), Adalimumab, Ibritumomab (negative control), and Tostiumomab (negative control) biosimilars. PVDF membrane was probed with 2 µg/mL of Anti- Adalimumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Adalimumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Specificity of Anti-Adalimumab (Anti-Idiotype) Antibody in ELISA Capture Assay.
Biotinylated biosimilars were serially diluted and incubated with Anti-Adalimumab (Anti-Idiotype) Antibody (Catalog # MAB9546, green line) coated at 1 μg/mL on a 96-well polystyrene plate. No cross-reactivity with Trastuzumab (black line) or Gemtuzumab (orange line) was observed.
Specificity of Anti-Adalimumab (Anti-Idiotype) Antibody in Direct ELISA.
A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Adalimumab (Anti-Idiotype) Antibody (Catalog # MAB9546, 333 ng/mL) detected Adalimumab. No cross-reactivity with Cetuximab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab was observed.Applications for Anti- Adalimumab (Anti-Idiotype) Antibody
Application
Recommended Usage
ELISA
Detects Adalimumab in direct ELISAs.
Western Blot
2 µg/mL
Sample: Adalimumab under non-reducing conditions only
Sample: Adalimumab under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Adalimumab
Additional Adalimumab Products
Product Documents for Anti- Adalimumab (Anti-Idiotype) Antibody
Product Specific Notices for Anti- Adalimumab (Anti-Idiotype) Antibody
For research use only
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