Anti- Gemtuzumab (Anti-Idiotype) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9617
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Multi-Species
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2263B
Product Specifications
Immunogen
Full length Gemtuzumab
Specificity
Detects Gemtuzumab in Western blots and direct ELISAs. No cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Trastuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Anti- Gemtuzumab (Anti-Idiotype) Antibody
Detection of Gemtuzumab by Western Blot.
Western blot shows Centuximab, Rituximab, Brentuximab, and Gemtuzumab biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti- Gemtuzumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9617) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Gemtuzumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Specificity of Anti-Gemtuzumab (Anti-Idiotype) Antibody in ELISA Capture Assay.
Biotinylated biosimilars were serially diluted and incubated with Anti-Gemtuzumab (Anti-Idiotype) Antibody (Catalog # MAB9617, green line) coated at 1 μg/mL on a 96-well polystyrene plate. No cross-reactivity with Rituximab (black line) or biotinylated IgG control (orange line) was observed.
Specificity of Anti-Gemtuzumab (Anti-Idiotype) Antibody in Direct ELISA.
A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Gemtuzumab (Anti-Idiotype) Antibody (Catalog # MAB9617, 333 ng/mL) detected Gemtuzumab. No cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Trastuzumab, Solanezumab, Teprotumumab, and Aducanumab was observed.Applications for Anti- Gemtuzumab (Anti-Idiotype) Antibody
Application
Recommended Usage
ELISA
Detects Gemtuzumab in direct ELISAs.
Western Blot
0.5 µg/mL
Sample: Gemtuzumab under non-reducing conditions
Sample: Gemtuzumab under non-reducing conditions
Reviewed Applications
Read 1 review rated 5 using MAB9617 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Gemtuzumab
Additional Gemtuzumab Products
Product Documents for Anti- Gemtuzumab (Anti-Idiotype) Antibody
Product Specific Notices for Anti- Gemtuzumab (Anti-Idiotype) Antibody
For research use only
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