Anti- Gemtuzumab (Anti-Idiotype) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB96171
Conjugate
Catalog #
Key Product Details
Species Reactivity
Multi-Species
Applications
ELISA, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 975155
Product Specifications
Immunogen
Full length Gemtuzumab
Specificity
Detects Gemtuzumab in Western blots and direct ELISAs. No cross-reactivity with Cetuximab, Ibritumomab, Rituximab, Adalimumab, Tositumomab, Brentuximab, Trastuzumab, Solanezumab, Teprotumumab, and Aducanumab is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Anti- Gemtuzumab (Anti-Idiotype) Antibody
Detection of Gemtuzumab by Western Blot.
Western blot shows Rituximab (negative control), Trastuzumab (negative control), Gemtuzumab, and Brentuximab (negative control) biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti-Gemtuzumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB96171) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody HAF008). A specific band was detected for Gemtuzumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of anti-Siglec-3/CD33 (Gemtuzumab) on U937 Human Cell Line by Flow Cytometry.
U937 Human histiocytic lymphoma cell line was stained with anti-Siglec-3/CD33 (Gemtuzumab) (MAB9635), then Anti-Gemtuzumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB96171, filled histogram) or Mouse IgG2AIsotype Control Antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).
Specificity of Anti-Gemtuzumab (Anti-Idiotype) Antibody in ELISA Capture Assay.
Biotinylated biosimilars were serially diluted and incubated with Anti-Gemtuzumab (Anti-Idiotype) Antibody (Catalog # MAB96171, green line) coated at 1 μg/mL on a 96-well polystyrene plate. No cross-reactivity with Rituximab (black line) or biotinylated IgG control (orange line) was observed.Applications for Anti- Gemtuzumab (Anti-Idiotype) Antibody
Application
Recommended Usage
ELISA
Detects Gemtuzumab in direct ELISAs.
Flow Cytometry
0.25 µg/106 cells
Sample: U937 Human cell line plus Anti-Siglec-3/CD33 (Gemtuzumab), Catalog # MAB9635
Sample: U937 Human cell line plus Anti-Siglec-3/CD33 (Gemtuzumab), Catalog # MAB9635
Western Blot
0.5 µg/mL
Sample: Gemtuzumab under non-reducing conditions only
Sample: Gemtuzumab under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Gemtuzumab
Additional Gemtuzumab Products
Product Documents for Anti- Gemtuzumab (Anti-Idiotype) Antibody
Product Specific Notices for Anti- Gemtuzumab (Anti-Idiotype) Antibody
For research use only
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