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AOF1 Antibody (5E10C1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52415

Novus Biologicals, part of Bio-Techne
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NBP2-52415
NBP2-52415-0.025mg

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5E10C1

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Purified recombinant fragment of human AOF1 (AA: 6-129) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AOF1 Antibody (5E10C1) - BSA Free

Western Blot: AOF1 Antibody (5E10C1)BSA Free [NBP2-52415]

Western Blot: AOF1 Antibody (5E10C1)BSA Free [NBP2-52415]

Western Blot: AOF1 Antibody (5E10C1) [NBP2-52415] - Western blot aAagainst human AOF1 (AA: 6-129) recombinant protein. (Expected MW is 40 kDa)
Immunocytochemistry/ Immunofluorescence: AOF1 Antibody (5E10C1) - BSA Free [NBP2-52415]

Immunocytochemistry/ Immunofluorescence: AOF1 Antibody (5E10C1) - BSA Free [NBP2-52415]

Immunocytochemistry/Immunofluorescence: AOF1 Antibody (5E10C1) [NBP2-52415] - Analysis of HeLa cells using AOF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry: AOF1 Antibody (5E10C1) - BSA Free [NBP2-52415]

Immunohistochemistry: AOF1 Antibody (5E10C1) - BSA Free [NBP2-52415]

Immunohistochemistry: AOF1 Antibody (5E10C1) [NBP2-52415] - Analysis of paraffin-embedded rectum cancer tissues using AOF1 mouse mAb with DAB staining.

Applications for AOF1 Antibody (5E10C1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AOF1

Long Name

Lysine-specific histone demethylase 1B

Alternate Names

C6orf193, EC 1.14.99.66, KDM1B, LSD2

Gene Symbol

KDM1B

Additional AOF1 Products

Product Documents for AOF1 Antibody (5E10C1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AOF1 Antibody (5E10C1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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