AOF1 Antibody (5E10C1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52415
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5E10C1
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified recombinant fragment of human AOF1 (AA: 6-129) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for AOF1 Antibody (5E10C1) - BSA Free
Western Blot: AOF1 Antibody (5E10C1)BSA Free [NBP2-52415]
Western Blot: AOF1 Antibody (5E10C1) [NBP2-52415] - Western blot aAagainst human AOF1 (AA: 6-129) recombinant protein. (Expected MW is 40 kDa)Immunocytochemistry/ Immunofluorescence: AOF1 Antibody (5E10C1) - BSA Free [NBP2-52415]
Immunocytochemistry/Immunofluorescence: AOF1 Antibody (5E10C1) [NBP2-52415] - Analysis of HeLa cells using AOF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.Immunohistochemistry: AOF1 Antibody (5E10C1) - BSA Free [NBP2-52415]
Immunohistochemistry: AOF1 Antibody (5E10C1) [NBP2-52415] - Analysis of paraffin-embedded rectum cancer tissues using AOF1 mouse mAb with DAB staining.Applications for AOF1 Antibody (5E10C1) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Application Notes
This antibody is Cytof ready.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AOF1
Long Name
Lysine-specific histone demethylase 1B
Alternate Names
C6orf193, EC 1.14.99.66, KDM1B, LSD2
Gene Symbol
KDM1B
Additional AOF1 Products
Product Documents for AOF1 Antibody (5E10C1) - BSA Free
Product Specific Notices for AOF1 Antibody (5E10C1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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