AP-2 beta/TFAP2B Antibody (3G5-1D11)

Novus Biologicals, part of Bio-Techne | Catalog # H00007021-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3G5-1D11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AP-2 beta Antibodies »

Product Specifications

Immunogen

TFAP2B (AAH37225, 1 a.a. ~ 449 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLWKLVENVKYEDIYEDRHDGVPSHSSRLSQLGSVSQGPYSSAPPLSHTPSSDFQPPYFPPPYQPLPYHQSQDPYSHVNDPYSLNPLHQPQQHPWGQRQRQEVGSEAGSLLPQPRAALPQLSGLDPRRDYHSVRRPDVLLHSAHHGLDAGMGDSLSLHGLGHPGMEDVQSVEDANNSGMNLLDQSVIKKVPVPPKSVTSLMMNKDGFLGGMSVNTGEVFCSVPGRLSLLSSTSKYKVTVGEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLRERLEKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYICETEFPAKAVSEYLNRQHTDPSDLHSRKNMLLATKQLCKEFTDLLAQDRTPIGNSRPSPILEPGIQSCLTHFSLITHGFGAPAICAALTALQNYLTEALKGMDKMFLNNTTTNRHTSGEGPGSKTGDKEEKHRK

Specificity

TFAP2B - transcription factor AP-2 beta (activating enhancer binding protein 2 beta)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for AP-2 beta/TFAP2B Antibody (3G5-1D11)

Western Blot: AP-2 beta/TFAP2B Antibody (3G5-1D11) [H00007021-M01]

Western Blot: AP-2 beta/TFAP2B Antibody (3G5-1D11) [H00007021-M01]

Western Blot: AP-2 beta/TFAP2B Antibody (3G5-1D11) [H00007021-M01] - Analysis of TFAP2B expression in transfected 293T cell line by TFAP2B monoclonal antibody (M01), clone 3G5-1D11.Lane 1: TFAP2B transfected lysate(49.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: AP-2 beta/TFAP2B Antibody (3G5-1D11) [H00007021-M01]

Immunocytochemistry/ Immunofluorescence: AP-2 beta/TFAP2B Antibody (3G5-1D11) [H00007021-M01]

Immunocytochemistry/Immunofluorescence: AP-2 beta/TFAP2B Antibody (3G5-1D11) [H00007021-M01] - Analysis of monoclonal antibody to TFAP2B on HeLa cell . Antibody concentration 10 ug/ml.

Applications for AP-2 beta/TFAP2B Antibody (3G5-1D11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AP-2 beta

This gene encodes a member of the AP-2 family of transcription factors. AP-2 proteins form homo- or hetero-dimers with other AP-2 family members and bind specific DNA sequences. They are thought to stimulate cell proliferation and suppress terminal differentiation of specific cell types during embryonic development. Specific AP-2 family members differ in their expression patterns and binding affinity for different promoters. This protein functions as both a transcriptional activator and repressor. Mutations in this gene result in autosomal dominant Char syndrome, suggesting that this gene functions in the differentiation of neural crest cell derivatives.

Long Name

Activating Enhancer-binding Protein 2 beta

Alternate Names

AP2 beta, CHAR, TFAP2B

Entrez Gene IDs

7021 (Human)

Gene Symbol

TFAP2B

OMIM

169100 (Human)

UniProt

AAH37225 (Human)

Additional AP-2 beta Products

Product Documents for AP-2 beta/TFAP2B Antibody (3G5-1D11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AP-2 beta/TFAP2B Antibody (3G5-1D11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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