Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all AP1GBP1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by the antibody maps to a region between residue 975 to 1025 of human Gamma-synergin using the numbering given in entry NP_009178.3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AP1GBP1 Antibody

Western Blot: AP1GBP1 Antibody [NBP2-36531]

Western Blot: AP1GBP1 Antibody [NBP2-36531]

Western Blot: AP1GBP1 Antibody [NBP2-36531] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Gamma-synergin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: AP1GBP1 Antibody [NBP2-36531]

Immunoprecipitation: AP1GBP1 Antibody [NBP2-36531]

Immunoprecipitation: AP1GBP1 Antibody [NBP2-36531] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated Gamma-synergin, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for AP1GBP1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: AP1GBP1

The AP1GBP1 gene encodes a protein that interacts with the gamma subunit of AP1 clathrin-adaptor complex. The AP1 complex is located at the trans-Golgi network and associates specific proteins with clathrin-coated vesicles. This encoded protein may act to connec

Alternate Names

AP1 gamma subunit binding protein 1, AP1 subunit gamma-binding protein 1, FLJ34482, gamma-synergin, MGC104959, synergin gamma, synergin, gamma, SYNGAP1GBP1adaptor-related protein complex 1 gamma subunit-binding protein 1

Gene Symbol

SYNRG

UniProt

NP_009178.3 (Human)

Additional AP1GBP1 Products

Product Documents for AP1GBP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AP1GBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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