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AP1S2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33101

Novus Biologicals, part of Bio-Techne
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NBP1-33101

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Chicken (99%), Chimpanzee (100%), Mouse (100%), Rat (100%), Rhesus Macaque (90%), Xenopus (98%), Zebrafish (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Full length human AP1S2 Recombinant protein.

Reactivity Notes

Xenopus laevis (98%).

Localization

Golgi apparatus, Cytoplasmic vesicle membrane, Membrane, clathrin-coated pit

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for AP1S2 Antibody

Immunocytochemistry/ Immunofluorescence: AP1S2 Antibody [NBP1-33101]

Immunocytochemistry/ Immunofluorescence: AP1S2 Antibody [NBP1-33101]

Immunocytochemistry/Immunofluorescence: AP1S2 Antibody [NBP1-33101] - Analysis of methanol-fixed HeLa, using AP1S2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
AP1S2 Antibody

Western Blot: AP1S2 Antibody [NBP1-33101] -

Western Blot: AP1S2 Antibody [NBP1-33101] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with AP1S2 antibody (NBP1-33101) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for AP1S2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AP1S2

Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as the small subunit of this complex and is a member of the adaptin protein family. Transcript variants utilizing alternative polyadenylation signals exist for this gene.

Alternate Names

adaptor-related protein complex 1, sigma 2 subunit, MGC:1902, SIGMA1B, X-linked 59

Gene Symbol

AP1S2

Additional AP1S2 Products

Product Documents for AP1S2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AP1S2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.