AP2A2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-68948

Novus Biologicals, part of Bio-Techne
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NBP1-68948
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all AP2A2 Antibodies »

Product Summary for AP2A2 Antibody

Immunogen

Synthetic peptides corresponding to AP2A2 (adaptor-related protein complex 2, alpha 2 subunit) The peptide sequence was selected from the C terminal of AP2A2 (NP_036437). Peptide sequence LAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTSAVSTPSPSADL. The peptide sequence for this immunogen was taken from within the described region.

Specificity

This product is specific to Subunit or Isoform: alpha-2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for AP2A2 Antibody

Western Blot: AP2A2 Antibody [NBP1-68948]

Western Blot: AP2A2 Antibody [NBP1-68948]

Western Blot: AP2A2 Antibody [NBP1-68948] - Human Lung lysate, concentration 0.2-1 ug/ml.

Applications for AP2A2 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AP2A2

AP2A2 is a component of the adaptor protein complex 2 (AP-2). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif .

Alternate Names

100 kDa coated vesicle protein C, Adapter-related protein complex 2 alpha-2 subunit, adaptin, alpha B, Adaptor protein complex AP-2 subunit alpha-2, adaptor-related protein complex 2, alpha 2 subunit, alpha2-adaptin, Alpha-adaptin C, alpha-adaptin C; Huntingtin interacting protein J, AP-2 complex subunit alpha-2, Clathrin assembly protein complex 2 alpha-C large chain, clathrin-associated/assembly/adaptor protein, large, alpha 2, DKFZP564D1864, HIP9ADTAB, Huntingtin yeast partner J, Huntingtin-interacting protein 9, Huntingtin-interacting protein J, HYPJCLAPA2, KIAA0899HIP-9, Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit

Entrez Gene IDs

161 (Human)

Gene Symbol

AP2A2

UniProt

O94973 (Human)

Additional AP2A2 Products

Product Documents for AP2A2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AP2A2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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