AP2M1 Antibody (10J8D1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15341

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-15341-100ul
NBP3-15341-20ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10J8D1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AP2M1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human AP2M1 (Q96CW1). KLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AP2M1 Antibody (10J8D1)

Western Blot: AP2M1 Antibody (10J8D1) [NBP3-15341]

Western Blot: AP2M1 Antibody (10J8D1) [NBP3-15341]

Western Blot: AP2M1 Antibody (10J8D1) [NBP3-15341] - Western blot analysis of extracts of various cell lines, using AP2M1 Rabbit mAb (NBP3-15341) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: AP2M1 Antibody (10J8D1) [NBP3-15341]

Immunocytochemistry/ Immunofluorescence: AP2M1 Antibody (10J8D1) [NBP3-15341]

Immunocytochemistry/Immunofluorescence: AP2M1 Antibody (10J8D1) [NBP3-15341] - Immunofluorescence analysis of U-251MG cells using AP2M1 Rabbit mAb (NBP3-15341) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for AP2M1 Antibody (10J8D1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AP2M1

AP2M1 is a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. It is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. It may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein.

Alternate Names

Adaptin-mu2, adaptor-related protein complex 2, mu 1 subunit, AP-2 mu chain, Clathrin assembly protein complex 2 medium chain, clathrin coat adaptor protein AP50, Clathrin coat assembly protein AP50, Clathrin coat-associated protein AP50, clathrin-associated/assembly/adaptor protein, medium 1, HA2 50 kDa subunit, KIAA0109, mu subunit, Plasma membrane adaptor AP-2 50 kDa protein, plasma membrane adaptor AP-2 50kDA protein

Gene Symbol

AP2M1

Additional AP2M1 Products

Product Documents for AP2M1 Antibody (10J8D1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for AP2M1 Antibody (10J8D1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.