AP2S1 Antibody (3E4)
Novus Biologicals, part of Bio-Techne | Catalog # H00001175-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3E4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
AP2S1 (AAH06337, 1 a.a. ~ 142 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MIRFILIQNRAGKTRLAKWYMQFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIIYRRYAGLYFCICVDVNDNNLAYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAGEIRETSQTKVLKQLLMLQSLE
Specificity
AP2S1 (3E4)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for AP2S1 Antibody (3E4)
![Western Blot: AP2S1 Antibody (3E4) [H00001175-M01]](https://resources.bio-techne.com/images/products/AP2S1-Antibody-3E4-Western-Blot-H00001175-M01-img0005.jpg "Western Blot: AP2S1 Antibody (3E4) [H00001175-M01]")
Western Blot: AP2S1 Antibody (3E4) [H00001175-M01]
Western Blot: AP2S1 Antibody (3E4) [H00001175-M01] - Analysis of AP2S1 expression in transfected 293T cell line by AP2S1 monoclonal antibody (M01), clone 3E4.Lane 1: AP2S1 transfected lysate(17 KDa).Lane 2: Non-transfected lysate.![Western Blot: AP2S1 Antibody (3E4) [H00001175-M01]](https://resources.bio-techne.com/images/products/AP2S1-Antibody-3E4-Western-Blot-H00001175-M01-img0003.jpg "Western Blot: AP2S1 Antibody (3E4) [H00001175-M01]")
Western Blot: AP2S1 Antibody (3E4) [H00001175-M01]
Western Blot: AP2S1 Antibody (3E4) [H00001175-M01] - Analysis of AP2S1 over-expressed 293 cell line, cotransfected with AP2S1 Validated Chimera RNAi ( Cat # H00001175-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AP2S1 monoclonal antibody (M01), clone 3E4 (Cat # H00001175-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: AP2S1 Antibody (3E4) [H00001175-M01] - Detection limit for recombinant GST tagged AP2S1 is 0.3 ng/ml as a capture antibody.
Applications for AP2S1 Antibody (3E4)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: AP2S1
Alternate Names
Adapter-related protein complex 2 sigma subunit, Adaptor protein complex AP-2 subunit sigma, adaptor-related protein complex 2, sigma 1 subunit, AP17HA2 17 kDa subunit, AP-2 complex subunit sigma, CLAPS2AP17-DELTA, Clathrin assembly protein 2 small chain, Clathrin coat assembly protein AP17, Clathrin coat-associated protein AP17, clathrin-associated/assembly/adaptor protein, small 2 (17kD), Plasma membrane adaptor AP-2 17 kDa protein, Sigma2-adaptin
Entrez Gene IDs
1175 (Human)
Gene Symbol
AP2S1
OMIM
602242 (Human)
UniProt
Additional AP2S1 Products
Product Documents for AP2S1 Antibody (3E4)
Product Specific Notices for AP2S1 Antibody (3E4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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