AP2S1 Antibody (JE47-37)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32041
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE47-37
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human AP2S1 aa 1-50 / 142. (Uniprot: P53680)
Localization
Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for AP2S1 Antibody (JE47-37)
Western Blot: AP2S1 Antibody (JE47-37) [NBP3-32041]
Western Blot: AP2S1 Antibody (JE47-37) [NBP3-32041] - Western blot analysis of AP2S1 on different lysates with Rabbit anti-AP2S1 antibody (NBP3-32041) at 1/1,000 dilution.Lane 1: K-562 cell lysate (30 ug/Lane)
Lane 2: MCF7 cell lysate (30 ug/Lane)
Lane 3: NIH/3T3 cell lysate (30 ug/Lane)
Lane 4: PC-12 cell lysate (30 ug/Lane)
Lane 5: Mouse spleen tissue lysate (40 ug/Lane)
Lane 6: Rat kidney tissue lysate (40 ug/Lane)
Predicted band size: 17 kDa
Observed band size: 14 kDa
Exposure time: 1 minute 6 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32041) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for AP2S1 Antibody (JE47-37)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AP2S1
Alternate Names
Adapter-related protein complex 2 sigma subunit, Adaptor protein complex AP-2 subunit sigma, adaptor-related protein complex 2, sigma 1 subunit, AP17HA2 17 kDa subunit, AP-2 complex subunit sigma, CLAPS2AP17-DELTA, Clathrin assembly protein 2 small chain, Clathrin coat assembly protein AP17, Clathrin coat-associated protein AP17, clathrin-associated/assembly/adaptor protein, small 2 (17kD), Plasma membrane adaptor AP-2 17 kDa protein, Sigma2-adaptin
Gene Symbol
AP2S1
Additional AP2S1 Products
Product Documents for AP2S1 Antibody (JE47-37)
Product Specific Notices for AP2S1 Antibody (JE47-37)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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