AP5Z1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92049

Novus Biologicals, part of Bio-Techne
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NBP1-92049-25ul
NBP1-92049
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AP5Z1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DDQWLNVQAFSMLRAWLLHSGPEGPGTLDTDDRSEQEGSTLSVISATSSAGRLLPPRERLREVAFEYCQRLIEQSNRRALRKGDSDLQKACLVEAVLVLDVLCRQDPSFLYRSLS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%). Human Reactivity reported in scientific literature (PMID: 26085577).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AP5Z1 Antibody

Western Blot: AP5Z1 Antibody [NBP1-92049]

Western Blot: AP5Z1 Antibody [NBP1-92049]

Western Blot: AP5Z1 Antibody [NBP1-92049] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: AP5Z1 Antibody [NBP1-92049]

Immunocytochemistry/ Immunofluorescence: AP5Z1 Antibody [NBP1-92049]

Immunocytochemistry/Immunofluorescence: AP5Z1 Antibody [NBP1-92049] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: AP5Z1 Antibody [NBP1-92049]

Immunohistochemistry-Paraffin: AP5Z1 Antibody [NBP1-92049]

Immunohistochemistry-Paraffin: AP5Z1 Antibody [NBP1-92049] - Staining of human stomach shows strong nuclear positivity in glandular cells.
View 1 more images

Applications for AP5Z1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AP5Z1

Alternate Names

KIAA0415, SPG48hypothetical protein LOC9907

Entrez Gene IDs

9907 (Human)

Gene Symbol

AP5Z1

UniProt

O43299 (Human)

Additional AP5Z1 Products

Product Documents for AP5Z1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AP5Z1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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