Apc4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77378

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Apc4 Antibodies »

Product Summary for Apc4 Antibody - BSA Free

Immunogen

Antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human APC4. The immunogen is located within amino acids 40 - 90 of APC4. Amino Acid Squence: WSFPPNENTGKEVT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Apc4 Antibody - BSA Free

Western Blot: Apc4 AntibodyBSA Free [NBP1-77378]

Western Blot: Apc4 AntibodyBSA Free [NBP1-77378]

Western Blot: Apc4 Antibody [NBP1-77378] - Analysis of APC4 in mouse liver tissue lysate with APC4 antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry-Paraffin: Apc4 Antibody - BSA Free [NBP1-77378]

Immunohistochemistry-Paraffin: Apc4 Antibody - BSA Free [NBP1-77378]

Immunohistochemistry-Paraffin: Apc4 Antibody [NBP1-77378] - Rat liver tissue with APC4 antibody at 5 ug/mL.

Applications for Apc4 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apc4

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC4 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC4, it is thought that APC4 associates with other APC/C components APC1, APC5, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.

Alternate Names

anaphase promoting complex subunit 4, anaphase-promoting complex subunit 4, APC4Cyclosome subunit 4

Gene Symbol

ANAPC4

UniProt

Q9UJX5 (Human)

Additional Apc4 Products

Product Documents for Apc4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Apc4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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