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Apelin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12282

Novus Biologicals, part of Bio-Techne
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NBP3-12282

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic peptide taken within amino acid region 25-77 on human Apelin protein.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for Apelin Antibody

Immunohistochemistry-Paraffin: Apelin Antibody [NBP3-12282]

Immunohistochemistry-Paraffin: Apelin Antibody [NBP3-12282]

Immunohistochemistry-Paraffin: Apelin Antibody [NBP3-12282] - Rat lung. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.
Immunohistochemistry-Paraffin: Apelin Antibody [NBP3-12282]

Immunohistochemistry-Paraffin: Apelin Antibody [NBP3-12282]

Immunohistochemistry-Paraffin: Apelin Antibody [NBP3-12282] - Rat small intestine. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

Applications for Apelin Antibody

Application
Recommended Usage

ELISA

1:50000

Immunohistochemistry

1:50-1:150

Immunohistochemistry-Paraffin

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: Apelin

Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei and is an endogenous ligand for APJ, a G protein-coupled orphan receptor which is an alternative coreceptor with CD4 for HIV-1. Apelin and APJ are ubiquitously expressed in peripheral tissues, with highest levels reported for heart and lungs, as well several regions within the central nervous system. The actions of apelin remain largely unknown: Apelin inhibits HIV-1 entry in cells coexpressing CD4 and APJ; the oral intake of Apelin in colostrum and breast milk could have a role in the modulation of the immune responses in neonates; more recent studies have also indicated a role for Apelin in the central control of body fluid homeostasis, by influencing AVP release and drinking behavior. In the cardiovascular system several actions of Apelin have been described, including endothelium-dependent vasodilatation, vasoconstriction through direct action on smooth muscle and positive inotropism.

Alternate Names

AGTRL1 Lligand, APEL, APLN, XNPEP2

Gene Symbol

APLN

Additional Apelin Products

Product Documents for Apelin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Apelin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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