Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 19F461

Concentration

0.5 mg/ml

View all APIP Antibodies »

Product Specifications

Immunogen

The antibody was developed against a full-length His-tagged recombinant APIP.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (91%) Use in Mouse reported in scientific literature (PMID:31263105).

Localization

Cytoplasm, Nucleus

Specificity

This monoclonal antibody can be used for detection of APIP and APIP2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for APIP Antibody (19F461)

Western Blot: APIP Antibody (19F461) [NB100-56567]

Western Blot: APIP Antibody (19F461) [NB100-56567]

Western Blot: APIP Antibody (19F461) [NB100-56567] - Analysis of APIP2 in (A) recombinant protein and (B) HeLa cell lysate using APIP antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Analysis of human lung cancer tissue section using APIP antibody (clone 19F461) at a concentration of 5 ug/ml. The representative image shows a nuclear and cytoplasmic staining pattern of APIP expression in lung cancer cells.
Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567]

Immunohistochemistry-Paraffin: APIP Antibody (19F461) [NB100-56567] - Formalin-fixed, paraffin-embedded adenocarcinoma of the breast stained with APIP antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
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Applications for APIP Antibody (19F461)

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APIP

The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis3. Recently, Cho et al have identified a new Apaf-1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.

Alternate Names

APAF1 interacting protein, APAF1-interacting protein, APIP2, CGI29, CGI-29, dJ179L10.2, EC 4.2.1.109, MMRP19, MTRu-1-P dehydratase, probable methylthioribulose-1-phosphate dehydratase

Entrez Gene IDs

51074 (Human); 56369 (Mouse); 508345 (Bovine)

Gene Symbol

APIP

UniProt

Q96GX9 (Human)

Additional APIP Products

Product Documents for APIP Antibody (19F461)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for APIP Antibody (19F461)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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