APIP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89039

Novus Biologicals, part of Bio-Techne
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NBP1-89039
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all APIP Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NTPEEKDLKDRMAHAMNEYPDSCAVLVRRHGVYVWGETWEKAKTMCECYDYLFDIAVSMKKVGLDPSQLPVGENGIV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for APIP Antibody

Western Blot: APIP Antibody [NBP1-89039]

Western Blot: APIP Antibody [NBP1-89039]

Western Blot: APIP Antibody [NBP1-89039] - Analysis in control (vector only transfected HEK293T lysate) and APIP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: APIP Antibody [NBP1-89039]

Immunohistochemistry-Paraffin: APIP Antibody [NBP1-89039]

Immunohistochemistry-Paraffin: APIP Antibody [NBP1-89039] - Staining of human kidney shows cytoplasmic and nuclear positivity in tubule cells.

Applications for APIP Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APIP

The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Recently, Cho et al (4) have identified a new Apaf-1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.

Alternate Names

APAF1 interacting protein, APAF1-interacting protein, APIP2, CGI29, CGI-29, dJ179L10.2, EC 4.2.1.109, MMRP19, MTRu-1-P dehydratase, probable methylthioribulose-1-phosphate dehydratase

Gene Symbol

APIP

Additional APIP Products

Product Documents for APIP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for APIP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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