APOBEC3G Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77206

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Multiplex Immunoassay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all APOBEC3G Antibodies »

Product Specifications

Immunogen

Antibody was raised against a synthetic peptide corresponding to 15 amino acids near the amino-terminus of human APOBEC3G. The immunogen is located within the first 50 amino acids of APOBEC3G. Amino Acid Squence: RNTVERMYRDTFSYN

Specificity

APOBEC3G antibody will also detect the APOBEC3F isoform.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for APOBEC3G Antibody - BSA Free

Western Blot: APOBEC3G AntibodyBSA Free [NBP1-77206]

Western Blot: APOBEC3G AntibodyBSA Free [NBP1-77206]

Western Blot: APOBEC3G Antibody [NBP1-77206] - Caco-2 cell lysate in the (A), absence and (B) presence of blocking peptide with APOBEC3G antibody at 5 ug/ml. .
Immunohistochemistry-Paraffin: APOBEC3G Antibody - BSA Free [NBP1-77206]

Immunohistochemistry-Paraffin: APOBEC3G Antibody - BSA Free [NBP1-77206]

Immunohistochemistry-Paraffin: APOBEC3G Antibody [NBP1-77206] - Staining of APOBEC3G expression in Caco-2 cell lysate in the (A), absence and (B) presence of blocking peptide with anti-APOBEC3G (NT) at 5 ug/ml.

Applications for APOBEC3G Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1 ug/ml

Immunohistochemistry-Paraffin

1 ug/ml

Western Blot

5 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APOBEC3G

The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5alpha which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.

Alternate Names

APOBEC-related cytidine deaminase, APOBEC-related protein, APOBEC-related protein 9, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G, ARCD, ARP-9, bK150C2.7, CEM-15, CEM15ARP9, dJ494G10.1, DNA dC->dU-editing enzyme APOBEC-3G, EC 3.5.4, EC 3.5.4.-, EC 3.5.4.5, FLJ12740DNA dC->dU editing enzyme, MDS019, phorbolin-like protein MDS019

Gene Symbol

APOBEC3G

UniProt

NP_068594 (Human)

Additional APOBEC3G Products

Product Documents for APOBEC3G Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for APOBEC3G Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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