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APOL3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06979

Novus Biologicals, part of Bio-Techne
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NBP1-06979

Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence ELTQIYQRLNPCHTH corresponding to C-Terminus according to NP_663615.1, NP_055164.1, NP_663616.1.

Specificity

This antibody is expected to recognize isoform 1 (NP_663615.1), isoform 2 (NP_055164.1, NP_085147.1, NP_663614.1) and isoform 3 (NP_663616.1, NP_663617.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for APOL3 Antibody

Western Blot: APOL3 Antibody [NBP1-06979]

Western Blot: APOL3 Antibody [NBP1-06979]

Western Blot: APOL3 Antibody [NBP1-06979] - NBP1-06979 (1ug/ml) staining of DAUDI lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for APOL3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:1000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in lysates of DAUDI and MOLT4 (calculated MW of 44.3 kDa band according to NP_663615.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: APOL3

This gene is a member of the apolipoprotein L gene family. The encoded protein is found in the cytoplasm, where it may affect the movement of lipids or allow the binding of lipids to organelles. In addition, expression of this gene is upregulated by tumor necrosis factor-alpha in endothelial cells lining the normal and atherosclerotic iliac artery and aorta. Six transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

APOLIII, apoL-III, apolipoprotein L, 3, apolipoprotein L3, Apolipoprotein L-III, CG12_1, CG121, CG12-1, TNF-inducible protein CG12-1

Entrez Gene IDs

80833 (Human)

Gene Symbol

APOL3

Additional APOL3 Products

Product Documents for APOL3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for APOL3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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