Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Western Blot

Label

Janelia Fluor 549

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2083A

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Apolipoprotein A-I/ApoA1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ApoA1
Asp25-Gln267
Accession # P02647

Specificity

Detects human ApoA1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Apolipoprotein A-I/ApoA1 Antibody (2083A) [Janelia Fluor® 549]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Apolipoprotein A-I/ApoA1

Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Additional Apolipoprotein A-I/ApoA1 Products

Product Documents for Apolipoprotein A-I/ApoA1 Antibody (2083A) [Janelia Fluor® 549]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Apolipoprotein A-I/ApoA1 Antibody (2083A) [Janelia Fluor® 549]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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