Apolipoprotein A-II/ApoA2 Antibody (6H2)

Immunohistochemistry: Apolipoprotein A-II/ApoA2 Antibody (6H2) [NBP3-26504] -

Immunohistochemistry: Apolipoprotein A-II/ApoA2 Antibody (6H2) [NBP3-26504] - Image of Apolipoprotein A-II/ApoA2 Antibody (6H2) diluted at 1:87.5 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot: Apolipoprotein A-II/ApoA2 Antibody (6H2) [NBP3-26504] -

Western Blot: Apolipoprotein A-II/ApoA2 Antibody (6H2) [NBP3-26504] - Positive Western Blot detected in: 293T whole cell lysate, A549 whole cell lysate.
All lanes: Apolipoprotein A-II/ApoA2 Antibody at 0.87ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 12 KDa
Observed band size: 12 KDa

Flow Cytometry: Apolipoprotein A-II/ApoA2 Antibody (6H2) [NBP3-26504] -

Flow Cytometry: Apolipoprotein A-II/ApoA2 Antibody (6H2) [NBP3-26504] - Overlay histogram showing A549 cells stained with Apolipoprotein A-II/ApoA2 Antibody (6H2) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.