Apolipoprotein F Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20882
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-KSYDLDPGAGSLE corresponding to C-Terminus according to NP_001629.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Apolipoprotein F Antibody
![Western Blot: Apolipoprotein F Antibody [NBP1-20882]](https://resources.bio-techne.com/images/products/Apolipoprotein-F-Antibody-Western-Blot-NBP1-20882-img0004.jpg "Western Blot: Apolipoprotein F Antibody [NBP1-20882]")
Western Blot: Apolipoprotein F Antibody [NBP1-20882]
Western Blot: Apolipoprotein F Antibody [NBP1-20882] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: Apolipoprotein F Antibody [NBP1-20882]](https://resources.bio-techne.com/images/products/Apolipoprotein-F-Antibody-Immunohistochemistry-Paraffin-NBP1-20882-img0003.jpg "Immunohistochemistry-Paraffin: Apolipoprotein F Antibody [NBP1-20882]")
Immunohistochemistry-Paraffin: Apolipoprotein F Antibody [NBP1-20882]
Immunohistochemistry-Paraffin: Apolipoprotein F Antibody [NBP1-20882] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for Apolipoprotein F Antibody
Application
Recommended Usage
Immunohistochemistry
2.5 - 3.8 ug/ml
Immunohistochemistry-Paraffin
2.5 - 3.8 ug/ml
Peptide ELISA
Detection limit 1:32000
Western Blot
0.3 - 1 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in human liver lysates (calculated MW of 35.4 kDa band according to NP_001629.1). IHC-P: Human liver shows staniing of droplets in the cytoplasm of hepatocytes.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Apolipoprotein F
Alternate Names
Apo-F, apolipoprotein F, DKFZp781G18150, Lipid transfer inhibitor protein, LTIP, MGC22520
Entrez Gene IDs
319 (Human)
Gene Symbol
APOF
UniProt
Additional Apolipoprotein F Products
Product Documents for Apolipoprotein F Antibody
Product Specific Notices for Apolipoprotein F Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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