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Apolipoprotein H/ApoH Antibody (JE37-02)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32044

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32044

Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE37-02

Concentration

1 mg/ml

Product Summary for Apolipoprotein H/ApoH Antibody (JE37-02)

Immunogen

Synthetic peptide within Human Apolipoprotein H/ApoH aa 71-120 / 345. (Uniprot: P02749)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Apolipoprotein H/ApoH Antibody (JE37-02)

Apolipoprotein H/ApoH Antibody (JE37-02)

Western Blot: Apolipoprotein H/ApoH Antibody (JE37-02) [NBP3-32044]

Western Blot: Apolipoprotein H/ApoH Antibody (JE37-02) [NBP3-32044] - Western blot analysis of Apolipoprotein H/ApoH on human liver tissue lysates with Rabbit anti-Apolipoprotein H/ApoH antibody (NBP3-32044) at 1/1,000 dilution.

Lysates/proteins at 40 ug/Lane.

Predicted band size: 38 kDa
Observed band size: 38/55 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32044) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for Apolipoprotein H/ApoH Antibody (JE37-02)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein H/ApoH

Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.

Alternate Names

APOH, B2G1, B2GPI, Beta, BG

Gene Symbol

APOH

Additional Apolipoprotein H/ApoH Products

Product Documents for Apolipoprotein H/ApoH Antibody (JE37-02)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Apolipoprotein H/ApoH Antibody (JE37-02)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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