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Apolipoprotein L2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90127

Novus Biologicals, part of Bio-Techne
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NBP1-90127
NBP1-90127-25ul

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LGVRVREEEAGTRVKENLPVWTVTGELQGKPLGNPAAGTMNPESSIFIEDYLKYFQDQVSRENLLQLLTDDEAWNGFVAAAELPRDEADELRKALNKLASHMVMKDKNRHDKDQQHRQWFLKEFPRLKRELEDHIRKLRALAEEVEQVHR

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24901046).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Apolipoprotein L2 Antibody

Western Blot: Apolipoprotein L2 Antibody [NBP1-90127]

Western Blot: Apolipoprotein L2 Antibody [NBP1-90127]

Western Blot: Apolipoprotein L2 Antibody [NBP1-90127] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-APOL2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: Apolipoprotein L2 Antibody [NBP1-90127]

Immunocytochemistry/ Immunofluorescence: Apolipoprotein L2 Antibody [NBP1-90127]

Immunocytochemistry/Immunofluorescence: Apolipoprotein L2 Antibody [NBP1-90127] - Staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Apolipoprotein L2 Antibody [NBP1-90127]

Immunohistochemistry-Paraffin: Apolipoprotein L2 Antibody [NBP1-90127]

Immunohistochemistry-Paraffin: Apolipoprotein L2 Antibody [NBP1-90127] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for Apolipoprotein L2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein L2

Apolipoprotein L2 is a member of the apolipoprotein L gene family. The encoded protein is found in the cytoplasm, where it may affect the movement of lipids or allow the binding of lipids to organelles. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Alternate Names

APOL3, ApoL-II, apolipoprotein L, 2, apolipoprotein L2, Apolipoprotein L-II

Entrez Gene IDs

23780 (Human)

Gene Symbol

APOL2

UniProt

Additional Apolipoprotein L2 Products

Product Documents for Apolipoprotein L2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Apolipoprotein L2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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