Apolipoprotein L2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90127
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LGVRVREEEAGTRVKENLPVWTVTGELQGKPLGNPAAGTMNPESSIFIEDYLKYFQDQVSRENLLQLLTDDEAWNGFVAAAELPRDEADELRKALNKLASHMVMKDKNRHDKDQQHRQWFLKEFPRLKRELEDHIRKLRALAEEVEQVHR
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 24901046).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Apolipoprotein L2 Antibody
Western Blot: Apolipoprotein L2 Antibody [NBP1-90127]
Western Blot: Apolipoprotein L2 Antibody [NBP1-90127] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-APOL2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.Immunocytochemistry/ Immunofluorescence: Apolipoprotein L2 Antibody [NBP1-90127]
Immunocytochemistry/Immunofluorescence: Apolipoprotein L2 Antibody [NBP1-90127] - Staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies & cytosol. Antibody staining is shown in green.Immunohistochemistry-Paraffin: Apolipoprotein L2 Antibody [NBP1-90127]
Immunohistochemistry-Paraffin: Apolipoprotein L2 Antibody [NBP1-90127] - Staining of human skeletal muscle shows no positivity in myocytes as expected.Applications for Apolipoprotein L2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Apolipoprotein L2
Alternate Names
APOL3, ApoL-II, apolipoprotein L, 2, apolipoprotein L2, Apolipoprotein L-II
Entrez Gene IDs
23780 (Human)
Gene Symbol
APOL2
UniProt
Additional Apolipoprotein L2 Products
Product Documents for Apolipoprotein L2 Antibody
Product Specific Notices for Apolipoprotein L2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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