Apolipoprotein L4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20883

Novus Biologicals, part of Bio-Techne
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NBP1-20883
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Apolipoprotein L4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ENLNELTHIHQSLK corresponding to C-Terminus according to NP_085146.2, NP_663693.1.

Epitope

C terminus

Specificity

This antibody is expected to recognize both reported isoforms (NP_085146.2 and NP_663693.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Apolipoprotein L4 Antibody

Western Blot: Apolipoprotein L4 Antibody [NBP1-20883]

Western Blot: Apolipoprotein L4 Antibody [NBP1-20883]

Western Blot: Apolipoprotein L4 Antibody [NBP1-20883] - Analysis of Apolipoprotein L4 in Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide using this antibody at 0.5 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Apolipoprotein L4 Antibody [NBP1-20883]

Immunohistochemistry-Paraffin: Apolipoprotein L4 Antibody [NBP1-20883]

Immunohistochemistry-Paraffin: Apolipoprotein L4 Antibody [NBP1-20883] - (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Apolipoprotein L4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1:1000
Application Notes
WB: Approx. 37 kDa band observed in human placenta lysates (calculated MW of 38.7 kDa band according to NP_085146.2). Two additional bands of unknown identity were also consistently observed at 50 kDa band and at 70 kDa. The band at 50 kDa band was successfully blocked by incubation with the immunizing peptide. The other band at 70 kDa band however was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when the antibody is used for other assays than WB.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Apolipoprotein L4

The protein encoded by this gene is a member of the apolipoprotein L family and may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Two transcript variants encoding two different isoforms have been found for this gene. Only one of the isoforms appears to be a secreted protein.

Alternate Names

apolipoprotein L, 4, apolipoprotein L4, Apolipoprotein L-IV, APOLIV, ApoL-IV

Entrez Gene IDs

80832 (Human)

Gene Symbol

APOL4

UniProt

NP_085146.2 (Human)

Additional Apolipoprotein L4 Products

Product Documents for Apolipoprotein L4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Apolipoprotein L4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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