Aprataxin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98661
![Western Blot: Aprataxin Antibody [NBP2-98661]](https://resources.bio-techne.com/images/products/Aprataxin-Antibody-Western-Blot-NBP2-98661-img0004.jpg "Western Blot: Aprataxin Antibody [NBP2-98661]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human Aprataxin fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Aprataxin Antibody
Western Blot: Aprataxin Antibody [NBP2-98661]
Western Blot: Aprataxin Antibody [NBP2-98661] - Anti-Aprataxin rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lane C: 293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa![Immunocytochemistry/ Immunofluorescence: Aprataxin Antibody [NBP2-98661]](https://resources.bio-techne.com/images/products/Aprataxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98661-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Aprataxin Antibody [NBP2-98661]")
Immunocytochemistry/ Immunofluorescence: Aprataxin Antibody [NBP2-98661]
Immunocytochemistry/Immunofluorescence: Aprataxin Antibody [NBP2-98661] - Immunofluorescence staining of Aprataxin in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Aprataxin polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Nucleus.![Immunohistochemistry-Paraffin: Aprataxin Antibody [NBP2-98661]](https://resources.bio-techne.com/images/products/Aprataxin-Antibody-Immunohistochemistry-Paraffin-NBP2-98661-img0003.jpg "Immunohistochemistry-Paraffin: Aprataxin Antibody [NBP2-98661]")
Immunohistochemistry-Paraffin: Aprataxin Antibody [NBP2-98661]
Immunohistochemistry-Paraffin: Aprataxin Antibody [NBP2-98661] - Immunochemical staining of human Aprataxin in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.Applications for Aprataxin Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aprataxin
Alternate Names
AOA1EAOH, AOAMGC1072, aprataxin, ataxia 1, early onset with hypoalbuminemia, AXA1, EC 3.-, EOAHA, FHA-HIT, FLJ20157, Forkhead-associated domain histidine triad-like protein
Gene Symbol
APTX
Additional Aprataxin Products
Product Specific Notices for Aprataxin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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