ARA54 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99042

Novus Biologicals, part of Bio-Techne
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NBP2-99042-100ul
NBP2-99042-50ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ARA54 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human ARA54.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ARA54 Antibody

Immunocytochemistry/ Immunofluorescence: ARA54 Antibody [NBP2-99042]

Immunocytochemistry/ Immunofluorescence: ARA54 Antibody [NBP2-99042]

Immunocytochemistry/Immunofluorescence: ARA54 Antibody [NBP2-99042] - Immunofluorescence staining of ARA54 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human ARA54 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.
Immunohistochemistry-Paraffin: ARA54 Antibody [NBP2-99042]

Immunohistochemistry-Paraffin: ARA54 Antibody [NBP2-99042]

Immunohistochemistry-Paraffin: ARA54 Antibody [NBP2-99042] - Immunochemical staining of human RNF14 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Applications for ARA54 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARA54

RNF14 is encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Long Name

Androgen Receptor Associated Protein 54

Alternate Names

HFB30, RNF14, TRIAD2

Gene Symbol

RNF14

Additional ARA54 Products

Product Documents for ARA54 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ARA54 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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