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ARC/NOL3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-257

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

A synthetic peptide made to residues 125-141 (PELQTLTRPGALRAPRR) of the human Nop30 protein.

Reactivity Notes

Reacts with residues 125-141 (PELQTLTRPGALRAPRR) of the human Nop30 protein. Reactivity to other species has not tested.

Specificity

This is specific for the human Nop30 protein (Gene names - ARC, NOP or NOL3).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARC/NOL3 Antibody - BSA Free

Western Blot: ARC/NOL3 AntibodyBSA Free [NB500-257]

Western Blot: ARC/NOL3 AntibodyBSA Free [NB500-257]

Western Blot: ARC/NOL3 Antibody [NB500-257] - Analysis of NB500-257 at 1:1000 dilution. Lane 1: NIH3T3 cells. Lane 2: NIH3T3 cells transfected with NOL3. Lane 3: Recombinant ARC. Lane 4: Recombinant NOL3.

Applications for ARC/NOL3 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:100-1:2000
Application Notes
This antibody has been tested in Western blot, where a band at ~25 kDa is seen. No other applications have been tested. Western blot - Use at 1:500 to 1:1,000 dilution. *The investigator should determine the optimal dilution for each assay. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARC

Nop30 (nucleolar protein of 30 kDa) is predominantly expressed in muscle and is present in the nucleoplasm and concentrated in nucleoli. The predominant nucleolar localization of Nop30 points to a potential role in RNA processing in muscle nucleoli. A CARD domain containing protein, designated ARC for apoptosis repressor with CARD is related to Nop30 with a different carboxyl terminus.

Long Name

Apoptosis Repressor with Caspase Recruitment Domain

Alternate Names

NOL3

Entrez Gene IDs

8996 (Human)

Gene Symbol

NOL3

OMIM

614937 (Human)

UniProt

Additional ARC Products

Product Documents for ARC/NOL3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ARC/NOL3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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