ARF1 Antibody (0C5B3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16782

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16782-100ul
NBP3-16782-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0C5B3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ARF1 Antibodies »

Product Summary for ARF1 Antibody (0C5B3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 70-150 of human ARF1 (P84077). GQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARF1 Antibody (0C5B3)

Western Blot: ARF1 Antibody (0C5B3) [NBP3-16782]

Western Blot: ARF1 Antibody (0C5B3) [NBP3-16782]

Western Blot: ARF1 Antibody (0C5B3) [NBP3-16782] - Western blot analysis of extracts of various cell lines, using ARF1 Rabbit mAb (NBP3-16782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: ARF1 Antibody (0C5B3) [NBP3-16782]

Immunocytochemistry/ Immunofluorescence: ARF1 Antibody (0C5B3) [NBP3-16782]

Immunocytochemistry/Immunofluorescence: ARF1 Antibody (0C5B3) [NBP3-16782] - Immunofluorescence analysis of NIH-3T3 cells using ARF1 Rabbit mAb (NBP3-16782) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for ARF1 Antibody (0C5B3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARF1

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.

Long Name

ADP-ribosylation factor 1

Alternate Names

ADP-ribosylation factor 1

Gene Symbol

ARF1

Additional ARF1 Products

Product Documents for ARF1 Antibody (0C5B3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ARF1 Antibody (0C5B3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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