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ARF3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31004

Novus Biologicals, part of Bio-Techne
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NBP1-31004

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Porcine (100%), Rat (100%), Sheep (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ARF3. The exact sequence is proprietary.

Localization

Golgi apparatus

Specificity

This product has been tested only for its reactivity with ARF3, however the sequence homology of the immunogen indicates a possible cross reactivity with ARF1, ARF5 and ARF6

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARF3 Antibody

Western Blot: ARF3 Antibody [NBP1-31004]

Western Blot: ARF3 Antibody [NBP1-31004]

Western Blot: ARF3 Antibody [NBP1-31004] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE ADP-ribosylation factor 3 antibody, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: ARF3 Antibody [NBP1-31004]

Immunocytochemistry/ Immunofluorescence: ARF3 Antibody [NBP1-31004]

Immunocytochemistry/Immunofluorescence: ARF3 Antibody [NBP1-31004] - Immunofluorescence analysis of methanol-fixed HeLa, using ARF3 antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: ARF3 Antibody [NBP1-31004]

Immunohistochemistry-Paraffin: ARF3 Antibody [NBP1-31004]

Immunohistochemistry-Paraffin: ARF3 Antibody [NBP1-31004] - Sample: Paraffin-embedded mouse fore brain. ARF3 antibody dilution: 1:500.

Applications for ARF3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARF3

ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns.

Alternate Names

ADP-ribosylation factor 3, small GTP binding protein

Entrez Gene IDs

377 (Human)

Gene Symbol

ARF3

UniProt

Additional ARF3 Products

Product Documents for ARF3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ARF3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.