ARF3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12541

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-12541-100ul
NBP3-12541-50ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ARF3 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human ARF3 (Uniprot#: P61204; Met1-Lys181).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ARF3 Antibody

Western Blot: ARF3 Antibody [NBP3-12541]

Western Blot: ARF3 Antibody [NBP3-12541]

Western Blot: ARF3 Antibody [NBP3-12541] - Lane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: Raji Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:20 kDaObserved band size:20 kDa
Immunocytochemistry/ Immunofluorescence: ARF3 Antibody [NBP3-12541]

Immunocytochemistry/ Immunofluorescence: ARF3 Antibody [NBP3-12541]

Immunocytochemistry/Immunofluorescence: ARF3 Antibody [NBP3-12541] - Staining of ARF3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ARF3 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunoprecipitation: ARF3 Antibody [NBP3-12541]

Immunoprecipitation: ARF3 Antibody [NBP3-12541]

Immunoprecipitation: ARF3 Antibody [NBP3-12541] - Lane A:0.5 mg HeLa Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg Raji Whole Cell LysateLane D:0.5 mg MCF7 Whole Cell Lysate4 uL anti- ARF3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti- ARF3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 19kDaObserved band size :19 kDa

Applications for ARF3 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

1-5ul/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARF3

ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns.

Alternate Names

ADP-ribosylation factor 3, small GTP binding protein

Gene Symbol

ARF3

Additional ARF3 Products

Product Documents for ARF3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ARF3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.