ARF4 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92502

Novus Biologicals, part of Bio-Techne
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NBP2-92502-0.02ml
NBP2-92502-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ARF4 Antibodies »

Product Summary for ARF4 Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ARF4 (NP_001651.1). MGLTISSLFSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDRIRPLWKHYFQNTQGLIFVVDSNDRERIQEVADELQKMLLVDELRDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARF4 Antibody - Azide and BSA Free

Western Blot: ARF4 AntibodyAzide and BSA Free [NBP2-92502]

Western Blot: ARF4 AntibodyAzide and BSA Free [NBP2-92502]

Western Blot: ARF4 Antibody [NBP2-92502] - Analysis of extracts of various cell lines, using ARF4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: ARF4 Antibody - Azide and BSA Free [NBP2-92502]

Immunocytochemistry/ Immunofluorescence: ARF4 Antibody - Azide and BSA Free [NBP2-92502]

Immunocytochemistry/Immunofluorescence: ARF4 Antibody [NBP2-92502] - Analysis of NIH/3T3 cells using ARF4 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: ARF4 Antibody - Azide and BSA Free [NBP2-92502]

Immunocytochemistry/ Immunofluorescence: ARF4 Antibody - Azide and BSA Free [NBP2-92502]

Immunocytochemistry/Immunofluorescence: ARF4 Antibody [NBP2-92502] - Analysis of C6 cells using ARF4 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for ARF4 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARF4

The ARF4 gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins thatstimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and asactivators of phospholipase

Alternate Names

ADP-ribosylation factor 4, ARF2ADP-ribosylation factor 2

Gene Symbol

ARF4

Additional ARF4 Products

Product Documents for ARF4 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ARF4 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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