ARFIP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15444

Novus Biologicals, part of Bio-Techne
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NBP2-15444
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (97%), Mouse (93%), Porcine (97%), Rat (91%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ARFIP1 Antibodies »

Product Summary for ARFIP1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARFIP1. The exact sequence is proprietary.

Reactivity Notes

Chicken (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARFIP1 Antibody

Western Blot: ARFIP1 Antibody [NBP2-15444]

Western Blot: ARFIP1 Antibody [NBP2-15444]

Western Blot: ARFIP1 Antibody [NBP2-15444] - Sample (30 ug of whole cell lysate) A: HL-60 10% SDS PAGE gel, diluted at 1:2000.
Immunohistochemistry-Paraffin: ARFIP1 Antibody [NBP2-15444]

Immunohistochemistry-Paraffin: ARFIP1 Antibody [NBP2-15444]

Immunohistochemistry-Paraffin: ARFIP1 Antibody [NBP2-15444] - Analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.

Applications for ARFIP1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARFIP1

ADP-ribosylation factors, or ARFs, enhance the ADP ribosyltransferase activity of cholera toxin and are implicated in vesicle transport between endoplasmic reticulum and the Golgi complex. Arfaptin 1 is recruited from the cytosol to Golgi membranes by ARFs in a guanosine 5'-O-(3-thiotriphosphate)-dependent and brefeldin A-sensitive manner but is not a constituent of coatomer. Arfaptin 1 binds to nonmyristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1, another class I ARF. It binds with lower affinity to ARF5, a class II ARF, and with very little affinity to ARF6, a class III ARF. POR1 (also designated arfaptin 2) was first isolated as a Rac 1 binding protein necessary for Rac mediated Actin polymerization and the subsequent formation of membrane ruffles and lamellipodia. POR1 has also been shown to interact with the ADP ribosylation factor ARF6, a GTPase that associates with the plasma membrane and intracellular endosome vesicles, in a GTP dependent manner. The association of POR1 with ARF6 stimulates induction of Actin polymerization. POR1 appears to play a regulatory role through multiple signaling pathways in the reorganization of the cytoskeletal structure.

Alternate Names

ADP-ribosylation factor interacting protein 1, ADP-ribosylation factor-interacting protein 1, arfaptin 1, arfaptin-1, HSU52521, MGC117369

Gene Symbol

ARFIP1

UniProt

P53367 (Human)

Additional ARFIP1 Products

Product Documents for ARFIP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ARFIP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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