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ARFIP2 Antibody (2B5)

Novus Biologicals, part of Bio-Techne | Catalog # H00023647-M01

Novus Biologicals, part of Bio-Techne
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H00023647-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2B5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ARFIP2 (AAH00392, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTDGILGKAATMEIPIHGNGEARQLPEDDGLEQDLQQVMVSGPNLNETSIVSGGYGGSGDGLIPTGSGRHPSHSTTPSGPGDEVARGIAGEKFDIVKKWGINTYKCTKQL

Reactivity Notes

Human. Other species not tested.

Localization

Cytoplasmic

Specificity

ARFIP2 - ADP-ribosylation factor interacting protein 2 (arfaptin 2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for ARFIP2 Antibody (2B5)

Western Blot: ARFIP2 Antibody (2B5) [H00023647-M01]

Western Blot: ARFIP2 Antibody (2B5) [H00023647-M01]

Western Blot: ARFIP2 Antibody (2B5) [H00023647-M01] - ARFIP2 monoclonal antibody (M01), clone 2B5 Analysis of ARFIP2 expression in IMR-32.
Sandwich ELISA: ARFIP2 Antibody (2B5) [H00023647-M01] - Detection limit for recombinant GST tagged ARFIP2 is 0.03 ng/ml as a capture antibody.

Applications for ARFIP2 Antibody (2B5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ARFIP2

ARFIP2 is a ubiquitously expressed protein implicated in mediating cross talk between RAC and ARF small GTPases. It has been shown that ARFIP2 binds specifically to GTP-bound ARF1 and ARF6, but binds to Rac-GTP and Rac-GDP with similar affinities. The X-ray structure of arfaptin reveals an elongated, crescent-shaped dimer of 3-helix coiled-coils. Structures of arfaptin with Rac bound to either GDP or the slowly hydrolysable analog GMPPNP show that the switch regions adopt similar conformations in both complexes.

Alternate Names

ADP-ribosylation factor interacting protein 2, ADP-ribosylation factor-interacting protein 2, arfaptin 2, arfaptin-2, Partner of RAC1, POR1partner of RAC1 (arfaptin 2), Protein POR1

Entrez Gene IDs

23647 (Human)

Gene Symbol

ARFIP2

OMIM

601638 (Human)

Additional ARFIP2 Products

Product Documents for ARFIP2 Antibody (2B5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ARFIP2 Antibody (2B5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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