Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3D4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Argininosuccinate Lyase Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ASL (NP_001020114) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

51.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free

Western Blot: Argininosuccinate Lyase Antibody (OTI3D4)Azide and BSA Free [NBP2-70213]

Western Blot: Argininosuccinate Lyase Antibody (OTI3D4)Azide and BSA Free [NBP2-70213]

Western Blot: Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free [NBP2-70213] - Analysis of HT29 cell lysate (35ug).
Immunohistochemistry: Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free [NBP2-70213]

Immunohistochemistry: Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free [NBP2-70213]

Immunohistochemistry: Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free [NBP2-70213] - Analysis of Carcinoma of Human liver tissue.
Western Blot: Argininosuccinate Lyase Antibody (OTI3D4)Azide and BSA Free [NBP2-70213]

Western Blot: Argininosuccinate Lyase Antibody (OTI3D4)Azide and BSA Free [NBP2-70213]

Western Blot: Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free [NBP2-70213] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Argininosuccinate Lyase.
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Applications for Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Argininosuccinate Lyase

Argininosuccinate Lyase encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described.

Alternate Names

argininosuccinase, argininosuccinate lyase, Arginosuccinase, ASAL, EC 4.3.2.1

Gene Symbol

ASL

Additional Argininosuccinate Lyase Products

Product Documents for Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Argininosuccinate Lyase Antibody (OTI3D4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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